Uptake and biochemical responses of mussels Mytilus galloprovincialis exposed to sublethal nickel concentrations.
Identifieur interne : 000370 ( Main/Exploration ); précédent : 000369; suivant : 000371Uptake and biochemical responses of mussels Mytilus galloprovincialis exposed to sublethal nickel concentrations.
Auteurs : Hajer Attig [Tunisie] ; Alessandro Dagnino ; Alessandro Negri ; Jamel Jebali ; Hamadi Boussetta ; Aldo Viarengo ; Francesco Dondero ; Mohamed BanniSource :
- Ecotoxicology and environmental safety [ 1090-2414 ] ; 2010.
English descriptors
- KwdEn :
- Animals, Catalase (metabolism), Environmental Pollutants (pharmacokinetics), Environmental Pollutants (toxicity), Gastric Mucosa (metabolism), Glutathione Transferase (metabolism), Lipid Peroxidation (drug effects), Malondialdehyde (metabolism), Metallothionein (metabolism), Mytilus (drug effects), Mytilus (metabolism), Nickel (pharmacokinetics), Nickel (toxicity), Oxidative Stress (drug effects), Principal Component Analysis, Time Factors.
- MESH :
- chemical , metabolism : Catalase, Glutathione Transferase, Malondialdehyde, Metallothionein.
- chemical , pharmacokinetics : Environmental Pollutants, Nickel.
- chemical , toxicity : Environmental Pollutants, Nickel.
- drug effects : Lipid Peroxidation, Mytilus, Oxidative Stress.
- metabolism : Gastric Mucosa, Mytilus.
- Animals, Principal Component Analysis, Time Factors.
Abstract
In the present study, mussel (Mytilus galloprovincialis) digestive gland oxidative stress biomarkers and detoxification responses to acute exposure to nickel (Ni) were investigated. Mussels were exposed to two sublethal concentrations of Ni (135 μg/L per animal (2.5 μM) and 770 μg/L per animal (13 μM)) for 24, 48, 72, 96 h and 8 days. Following biological responses were measured: (1) glutathione S-transferase (GST) activity as a phase II conjugation enzyme, (2) catalase activity as antioxidant response, (3) malondialdehyde accumulation (MDA) as lipid peroxydation marker and metallothionein as specific response to metals exposure. The cholinergic system was evaluated using the acetylcholinesterase activity (AChE). Moreover, Ni uptakes during the exposure periods were assessed and the uptake rate constant determined. A correlation matrix (CM) between the investigated biomarkers and a principal component analysis (PCA) were achieved for the two tested concentrations. The Ni-uptake constant was higher in animals exposed to the lowest concentration. The CM and the PCA showed a time-dependent effect of the Ni exposure on the investigated biomarkers being more pronounced in animals exposed to the highest Ni concentration. While AChE showed a significant increase after 48 h and a further return to control values in the lowest concentration, it was drastically maintained inhibited in the highest concentration. Our data provided clues about the occurrence of different toxicokinetics and toxicodynamics of two Ni sublethal concentrations in an ecologically relevant organism.
DOI: 10.1016/j.ecoenv.2010.08.007
PubMed: 20800282
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000099
- to stream PubMed, to step Curation: 000099
- to stream PubMed, to step Checkpoint: 000099
- to stream Ncbi, to step Merge: 000046
- to stream Ncbi, to step Curation: 000046
- to stream Ncbi, to step Checkpoint: 000046
- to stream Main, to step Merge: 000379
- to stream Main, to step Curation: 000370
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Uptake and biochemical responses of mussels Mytilus galloprovincialis exposed to sublethal nickel concentrations.</title><author><name sortKey="Attig, Hajer" sort="Attig, Hajer" uniqKey="Attig H" first="Hajer" last="Attig">Hajer Attig</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Biochemistry and Environmental Toxicology, Higher Institute of Agronomy, ISA, 4042, Chott Mariem, Sousse, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Biochemistry and Environmental Toxicology, Higher Institute of Agronomy, ISA, 4042, Chott Mariem, Sousse</wicri:regionArea><wicri:noRegion>Sousse</wicri:noRegion></affiliation></author><author><name sortKey="Dagnino, Alessandro" sort="Dagnino, Alessandro" uniqKey="Dagnino A" first="Alessandro" last="Dagnino">Alessandro Dagnino</name></author><author><name sortKey="Negri, Alessandro" sort="Negri, Alessandro" uniqKey="Negri A" first="Alessandro" last="Negri">Alessandro Negri</name></author><author><name sortKey="Jebali, Jamel" sort="Jebali, Jamel" uniqKey="Jebali J" first="Jamel" last="Jebali">Jamel Jebali</name></author><author><name sortKey="Boussetta, Hamadi" sort="Boussetta, Hamadi" uniqKey="Boussetta H" first="Hamadi" last="Boussetta">Hamadi Boussetta</name></author><author><name sortKey="Viarengo, Aldo" sort="Viarengo, Aldo" uniqKey="Viarengo A" first="Aldo" last="Viarengo">Aldo Viarengo</name></author><author><name sortKey="Dondero, Francesco" sort="Dondero, Francesco" uniqKey="Dondero F" first="Francesco" last="Dondero">Francesco Dondero</name></author><author><name sortKey="Banni, Mohamed" sort="Banni, Mohamed" uniqKey="Banni M" first="Mohamed" last="Banni">Mohamed Banni</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20800282</idno><idno type="pmid">20800282</idno><idno type="doi">10.1016/j.ecoenv.2010.08.007</idno><idno type="wicri:Area/PubMed/Corpus">000099</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000099</idno><idno type="wicri:Area/PubMed/Curation">000099</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000099</idno><idno type="wicri:Area/PubMed/Checkpoint">000099</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000099</idno><idno type="wicri:Area/Ncbi/Merge">000046</idno><idno type="wicri:Area/Ncbi/Curation">000046</idno><idno type="wicri:Area/Ncbi/Checkpoint">000046</idno><idno type="wicri:Area/Main/Merge">000379</idno><idno type="wicri:Area/Main/Curation">000370</idno><idno type="wicri:Area/Main/Exploration">000370</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Uptake and biochemical responses of mussels Mytilus galloprovincialis exposed to sublethal nickel concentrations.</title><author><name sortKey="Attig, Hajer" sort="Attig, Hajer" uniqKey="Attig H" first="Hajer" last="Attig">Hajer Attig</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Biochemistry and Environmental Toxicology, Higher Institute of Agronomy, ISA, 4042, Chott Mariem, Sousse, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Biochemistry and Environmental Toxicology, Higher Institute of Agronomy, ISA, 4042, Chott Mariem, Sousse</wicri:regionArea><wicri:noRegion>Sousse</wicri:noRegion></affiliation></author><author><name sortKey="Dagnino, Alessandro" sort="Dagnino, Alessandro" uniqKey="Dagnino A" first="Alessandro" last="Dagnino">Alessandro Dagnino</name></author><author><name sortKey="Negri, Alessandro" sort="Negri, Alessandro" uniqKey="Negri A" first="Alessandro" last="Negri">Alessandro Negri</name></author><author><name sortKey="Jebali, Jamel" sort="Jebali, Jamel" uniqKey="Jebali J" first="Jamel" last="Jebali">Jamel Jebali</name></author><author><name sortKey="Boussetta, Hamadi" sort="Boussetta, Hamadi" uniqKey="Boussetta H" first="Hamadi" last="Boussetta">Hamadi Boussetta</name></author><author><name sortKey="Viarengo, Aldo" sort="Viarengo, Aldo" uniqKey="Viarengo A" first="Aldo" last="Viarengo">Aldo Viarengo</name></author><author><name sortKey="Dondero, Francesco" sort="Dondero, Francesco" uniqKey="Dondero F" first="Francesco" last="Dondero">Francesco Dondero</name></author><author><name sortKey="Banni, Mohamed" sort="Banni, Mohamed" uniqKey="Banni M" first="Mohamed" last="Banni">Mohamed Banni</name></author></analytic><series><title level="j">Ecotoxicology and environmental safety</title><idno type="eISSN">1090-2414</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Catalase (metabolism)</term><term>Environmental Pollutants (pharmacokinetics)</term><term>Environmental Pollutants (toxicity)</term><term>Gastric Mucosa (metabolism)</term><term>Glutathione Transferase (metabolism)</term><term>Lipid Peroxidation (drug effects)</term><term>Malondialdehyde (metabolism)</term><term>Metallothionein (metabolism)</term><term>Mytilus (drug effects)</term><term>Mytilus (metabolism)</term><term>Nickel (pharmacokinetics)</term><term>Nickel (toxicity)</term><term>Oxidative Stress (drug effects)</term><term>Principal Component Analysis</term><term>Time Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Catalase</term><term>Glutathione Transferase</term><term>Malondialdehyde</term><term>Metallothionein</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Environmental Pollutants</term><term>Nickel</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Environmental Pollutants</term><term>Nickel</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lipid Peroxidation</term><term>Mytilus</term><term>Oxidative Stress</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Gastric Mucosa</term><term>Mytilus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Principal Component Analysis</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the present study, mussel (Mytilus galloprovincialis) digestive gland oxidative stress biomarkers and detoxification responses to acute exposure to nickel (Ni) were investigated. Mussels were exposed to two sublethal concentrations of Ni (135 μg/L per animal (2.5 μM) and 770 μg/L per animal (13 μM)) for 24, 48, 72, 96 h and 8 days. Following biological responses were measured: (1) glutathione S-transferase (GST) activity as a phase II conjugation enzyme, (2) catalase activity as antioxidant response, (3) malondialdehyde accumulation (MDA) as lipid peroxydation marker and metallothionein as specific response to metals exposure. The cholinergic system was evaluated using the acetylcholinesterase activity (AChE). Moreover, Ni uptakes during the exposure periods were assessed and the uptake rate constant determined. A correlation matrix (CM) between the investigated biomarkers and a principal component analysis (PCA) were achieved for the two tested concentrations. The Ni-uptake constant was higher in animals exposed to the lowest concentration. The CM and the PCA showed a time-dependent effect of the Ni exposure on the investigated biomarkers being more pronounced in animals exposed to the highest Ni concentration. While AChE showed a significant increase after 48 h and a further return to control values in the lowest concentration, it was drastically maintained inhibited in the highest concentration. Our data provided clues about the occurrence of different toxicokinetics and toxicodynamics of two Ni sublethal concentrations in an ecologically relevant organism.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Banni, Mohamed" sort="Banni, Mohamed" uniqKey="Banni M" first="Mohamed" last="Banni">Mohamed Banni</name><name sortKey="Boussetta, Hamadi" sort="Boussetta, Hamadi" uniqKey="Boussetta H" first="Hamadi" last="Boussetta">Hamadi Boussetta</name><name sortKey="Dagnino, Alessandro" sort="Dagnino, Alessandro" uniqKey="Dagnino A" first="Alessandro" last="Dagnino">Alessandro Dagnino</name><name sortKey="Dondero, Francesco" sort="Dondero, Francesco" uniqKey="Dondero F" first="Francesco" last="Dondero">Francesco Dondero</name><name sortKey="Jebali, Jamel" sort="Jebali, Jamel" uniqKey="Jebali J" first="Jamel" last="Jebali">Jamel Jebali</name><name sortKey="Negri, Alessandro" sort="Negri, Alessandro" uniqKey="Negri A" first="Alessandro" last="Negri">Alessandro Negri</name><name sortKey="Viarengo, Aldo" sort="Viarengo, Aldo" uniqKey="Viarengo A" first="Aldo" last="Viarengo">Aldo Viarengo</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Attig, Hajer" sort="Attig, Hajer" uniqKey="Attig H" first="Hajer" last="Attig">Hajer Attig</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/NickelMaghrebV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000370 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000370 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= NickelMaghrebV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:20800282
|texte= Uptake and biochemical responses of mussels Mytilus galloprovincialis exposed to sublethal nickel concentrations.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20800282" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a NickelMaghrebV1
| This area was generated with Dilib version V0.6.27. |  |